Chief Justice John Roberts scolded the House managers and the president's legal team shortly after 1 a.m. Wednesday.

\"Those addressing the Senate should remember where they are,\" he said.

Sen. Susan Collins, R-Maine, one of the moderates who backed a single Democratic amendment on Tuesday night and is open to calling witnesses later in the trial, was spotted sending a note to the parliamentarian that raised concerns about Nadler's tone while he made his comments on the floor.

Democratic Sen. Chris Murphy of Connecticut tweeted that a Republican senator approached him, and warned that Democrats' strategy of forcing amendment votes late into the night Tuesday \"might scare off\" Republicans open to supporting witnesses later in the trial.

This is a tried and true Republican tactic. They used to do this all the time w Pelosi - claim they they were voting a particular way because she was mean to them.

It’s BS - just a way to try to build cover for a reckless vote. https://t.co/oNL8hPMhY4

— Chris Murphy (@ChrisMurphyCT) January 22, 2020

\"If they're trying to win the argument that's probably not the best way to go about it,\" Sen. John Thune, R-S.D., told reporters.

\"PHOTO:
ABC News
PHOTO: Chief Justice of the Supreme Court John Roberts addresses the Senate on the second day of the impeachment trial against President Donald Trump at the Capitol, Jan. 22, 2020.
>

Some Democrats appeared to acknowledge that emotions ran too high on both sides.

\"I think that the chief justice’s message was heard loud and clear, and it will affect the tone going forward,\" said Sen. Richard Blumenthal, D-Conn., who also criticized the Trump legal team's comments.

\"The Trump team had more table pounding and shouting than real facts. They channeled their inner-Trump,\" he said. \"Their appeal was to their base. That's what ought to alienate my Republican colleagues because that's not the American people.\"

Speaking ahead of opening arguments on Wednesday, Rep. Adam Schiff, D-Calif, the lead House manager, said Democrats would \"try to keep focused on the facts.\"

\"PHOTO:
Mandel Ngan/AFP/Getty Images
PHOTO: Lead House Manager Adam Schiff speaks to the press at the Capitol in Washington, D.C., Jan. 22, 2020.
>

\"The president's team would like nothing more than to provoke a bitter conflict. We're not going to let them,\" he said when asked about the reprimand from Roberts.

Schiff did not answer a question, initially directed to Nadler, about GOP criticism of Nadler's remarks.

ABC News' Allison Pecorin and Sarah Kolinovsky contributed to this report.

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