This was the first nighttime splashdown of a U.S.-crewed spacecraft since Apollo 8's return on Dec. 27, 1968, in the Pacific Ocean.

\"Recovery vessels are on the way to hoist the Crew Dragon Resilience spacecraft out of the ocean and place it inside the Dragon nest aboard,\" NASA tweeted early on Sunday.

LIVE: Watch Crew Dragon Resilience undock from the @Space_Station. NASA’s @SpaceX Crew-1 astronauts are scheduled to splash down in the Gulf of Mexico at 2:57am ET (6:57 UT). https://t.co/3c0kGUoCEJ

— NASA (@NASA) May 2, 2021

Three NASA astronauts -- Michael Hopkins, Victor Glover and Shannon Walker -- and Soichi Noguchi of the Japan Aerospace Exploration Agency are making the journey home. They launched into orbit Nov. 15, 2020, in what was the first operational crewed flight for the Crew Dragon capsule as part of NASA's commercial crew mission, and SpaceX's second-ever crewed flight to space.

\"PHOTO:
Aubrey Gemignani/UPI via Shutterstock
PHOTO: NASA crew-2 walks out for launch from the Kennedy Space Center, Fla., April 23, 2021.
>

For the past six months, the astronauts have worked on several experiments, including producing food in space by growing radishes in different types of light and soils. They also studied tissue chips that mimic human organs to learn more about how microgravity affects human health and diseases.

The crew was in \"great health\" for the return flight, NASA said. Ahead of Saturday's undocking, they were packing science freezers filled with research samples, personal items and emergency hardware in the Resilience spacecraft.

\"Coming home soon!\" Hopkins, the Crew-1 Dragon resilience commander, tweeted hours before the undocking. \"It's been a great mission with great teams both on the ground and on @Space_Station.\"

Coming home soon! It's been a great mission with great teams both on the ground and on @Space_Station. Here are some of my favorite memories from the past 6 and a half months on-orbit… pic.twitter.com/wRL9YQet4J

— Mike Hopkins (@Astro_illini) May 1, 2021

Glover, the crew's pilot, expressed \"gratitude, wonder, connection\" after celebrating his 45th birthday in space on Friday.

\"This orbiting laboratory is a true testament to what we can accomplish when we work together as a team,\" he said on Twitter. \"Crew-1 is ready for our ride home!\"

Gratitude, wonder, connection. I'm full of and motivated by these feelings on my birthday, as my first mission to space comes to an end. This orbiting laboratory is a true testament to what we can accomplish when we work together as a team. Crew-1 is ready for our ride home! pic.twitter.com/CC6MajkZ1E

— Victor Glover (@AstroVicGlover) April 30, 2021

When they landed, the Crew-1 astronauts will have spent 168 days in space. Upon splashdown, they will return to Houston. The Dragon spacecraft will be recovered and return to Cape Canaveral, where it will be inspected and refurbished for future missions.

\"PHOTO:
Joe Skipper/Reuters
PHOTO: A SpaceX Falcon 9 rocket, with the Crew Dragon capsule, is launched carrying four astronauts on a NASA commercial crew mission to the International Space Station at Kennedy Space Center in Cape Canaveral, Fla., April 23, 2021.
>

Crew-1 was the first of six crewed missions NASA and SpaceX plan to fly as part of NASA's commercial crew mission.

The second mission in the program launched on April 23 from the Kennedy Space Center in Cape Canaveral, Florida, with four astronauts aboard the Crew Dragon Endeavor spacecraft, the same one used during the first-ever crewed SpaceX flight last May.

The spacecraft docked at the International Space Station on April 24, after about a 23-hour ride. The Crew-2 astronauts will remain in space until October.

The flights mark a yearslong effort between NASA and the private sector to bring launch capabilities back to U.S. soil and end a dependency on Russia for trips to the International Space Station.

The Crew Dragon is the first spacecraft since the Space Shuttle to be certified by NASA to carry humans.

ABC News' Jon Haworth contributed to this report.

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